William or Guillaume Chambrelan, Peter Chamberlen the younger's father lived in Paris, France. According to the 1634 Visitation of London which was attested by his grandson Dr. Peter Chamberlen, William was a younger son of a Chamberlayne of Tankerville, Normandy.

Peter Chamberlen, who was the father of Hugh Chamberlen the elder was born to Peter Chamberlen the younger and Sara (nee de Laune) Chamberlen. He was baptised at the French Huguenot church on Threadneedle Street in London on 12^th^ May 1601

 I Peter Chambrelan the yonger of London Surgeon being Sicke of bodie butt of good and perfect mynde and memorie thankes be therefore given to God Doe make and ordaine this my last will and Testament in manner and forme followinge That is to saie